AFAIK you need all of
d-i keyboard-configuration/variant select English (US)d-i console-keymaps-at/keymap select usd-i keyboard-configuration/xkb-keymap select us
and possibly
console-setup/ask_detect=false console-setup/layoutcode=us keyboard-configuration/xkb-keymap=us
on your append line of txt.cfg (or whatever isolinux config)